Introduction
Nanodsics are synthetic membrane cell mimic system composed of a double lipid bilayer stabilised by a membrane scaffold protein (MSP). This system allow the stabilization of the hydrophobic section of the membrane protein.
Nuclera curated 8 GPCR-specific pre-assembled nanodiscs tailored for the synthesis and stabilization of GPCRs in cell free system. The use of nanodiscs bypass the use of detergent for the solubilization of the membrane protein, maintaining the lipid interaction crytical for stability.
To maximise the success of stabilization of the synthesised membrane protein, you should systematically evaluate your choice of MSP and lipid ratios during the selection process.
MSP selection
Nuclera nanodiscs selection varies between MSP1D1 and MSP1E3D1. The different size of the MSP can stabilise differetnly the varies geometries and loop structures.
The difference between the 2 MSP is the specific diameters: 9–10 nm (MSP1D1) and 12–14 nm (MSP1E3D1).
Lipids selection
Nuclera nanodiscs offer the selection of following lipids:
- DOPG: The proven standard for cryo-EM1,2. Anionic environment validated for general solubility and insertion.
- DOPG/Cardiolipin: Enhanced stabilization. Highly anionic proven to improve ligand binding fraction and stabilize complex targets3.
- POPC/POPS: Native-like mimic. A physiologically relevant zwitterionic/ anionic charge balance.
- POPC/POPS/Cholesterol: Essential Functionality. Critical for cholesterol-dependent GPCRs and proven to enrich incorporation of complex GPCRs4.
- Merino, F. et al. Structure 33, 1867 (2025). 2. Köck, Z. et al. Nat. Commun. 15, 1831 (2024). 3. Köck, Z., et al. J. Mol. Biol. 434, 167687 (2022). 4. Roy, J., et al. Biochemistry 54, 6299 (2015).
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